Anyone have any good ideas?

When ever I try to access anything on the E drive (guess where most of my music and movies are?) it starts using 100% of the memory, everything slows to a dead crawl and even stops…I have ran all my defrags, my virus scanners, and my adware/spyware detectors/cleaners…I need to find a tool to see if I can find out what the problem is, and if I can, fix it….If I have to reformat the 250, I’m hosed…again!

Any recommendataions?